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- 2025年07月14日
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文献和实验Purification Mix. 2. Preparing the Lysate Follow the procedure below to prepare a lysate from the 50-100 µl blood sample. 1) Transfer the 50-100 µl blood sample to a sterile microcentrifuge tube or a 96 x 2 ml deep well plate
Extraction of genomic DNA from whole blood
should be sterile. Buffer A should be autoclaved prior to addition of Triton-X-100. Sterile filtering of solutions instead of autoclaving is a better option. Procedure 1.Add 1 volume of buffer A to 1 volume of blood and 2 volumes of cold, sterile, distilled
Harvesting lymphocytes from Peripheral Blood
from the whole blood ・ Add 250ml of DMEM with 10% Fetal Goat Serum(FGS) to 250ml of whole blood to block nonspecific binding ・ Incubate at 4°C for 10 minutes ・ Add 1mg of appropriate antibodies to the specimen (usually about a million
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