Goat blood, sterile Citrated

Human Peripheral Blood Mononuclear Cell Preparation

  This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained from a blood bank). Platelets, red blood cells, and neutrophils are largely depleted

DNA Extraction from Blood

Purification Mix. 2. Preparing the Lysate Follow the procedure below to prepare a lysate from the 50-100 µl blood sample. 1) Transfer the 50-100 µl blood sample to a sterile microcentrifuge tube or a 96 x 2 ml deep well plate

Extraction of genomic DNA from whole blood

should be sterile. Buffer A should be autoclaved prior to addition of Triton-X-100. Sterile filtering of solutions instead of autoclaving is a better option. Procedure 1.Add 1 volume of buffer A to 1 volume of blood and 2 volumes of cold, sterile, distilled