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文献和实验Purification of Endotoxin Free mAbs
is applicable to most rat and mouse IgG antibodies (not IgM), although individual monoclonal antibodies may require modification of either the pH or ionic strength for optimal purification. Cation exchange using Fast Flow S-Sepharose (Sigma S 1264) at pH
Cloning of Variable Domains from Mouse Hybridoma by PCR
of thoroughly validated oligonucleotide primers for a PCR procedure to isolate and clone the DNA of antibody Fv fragments (antigen binding region) from mouse and rat hybridoma cell lines. The protocol includes a colony staining assay to identify E. coli clones
Evaluation of Immunogenicity of Rat ES-Cell Derived Endothelial Cells
would enable their general use in allogeneic settings and therefore supply an unrestricted source. Based on conflicting data in terms of immunogenicity published for mouse and human ES-derived cells, the rat model was used to complement the knowledge
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