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文献和实验TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
Real-Time RT-PCR for CTG Repeat-Containing Genes
in this disorder. Real-time quantitative reverse transcription-polymerase chain reaction, or TaqMan, is a very sensitive method that enables quantification of expression levels of genes from small amounts of tissue and lowly expressed genes. Because data
在RT-PCR中避免DNA污染（Avoiding DNA Contamination in R
was isolated according to protocol by five different methods. 0.5 µg RNA was used in RT-PCR reactions with Ambion's RETROscript® Kit. PCR reactions were performed with 5 µg RNA. 10 µl of each reaction was electrophoresed on a 2% agarose gel and stained
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