STAT3基因敲除质粒

产品简介：

pLenti-STAT3-sgRNA (STAT3基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。

本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。

图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。

本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。

本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。

碧云天同时提供基于CRISPR/Cas9技术的STAT3基因敲除的质粒(L00456 pLenti-STAT3-sgRNA)、慢病毒(L00457 STAT3 Knockout Lentivirus)、HEK293T细胞(L00458 STAT3 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L00459 STAT3 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L00460 STAT3 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。

STAT3基因的基本信息如下：

Species	Gene Symbol	Gene ID	GenBank Accession	Transcript
Human	STAT3	6774	BC014482	NM_139276|NM_003150

 

About the gene
Official Symbol	STAT3
Previous Symbol	-
Official Full Name	signal transducer and activator of transcription 3
Synonyms	APRF
Location	17q21.2
Gene Type	protein-coding gene
Uniprot ID	P40763
Pathway/Library	cAMP & Ca Signaling Pathway Related Genes Library; Diabetes Related Genes Library
Gene Summary	The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described.