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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°-20°C
- 保质期:
1-3年
- 库存:
999
- 供应商:
亦高生物
- CAS号:
9002-93-1
- 规格:
T9284-500ML

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文献和实验Immunohistochemisty-Enzymatic Protocol
. Immunohistochem. Mol. Morphol. 8:236. Tissue Fixation and Mounting - Cryostat Sections Fix the tissue by vascular perfusion with 500 - 700 mL of Fixative. Perfuse the animal with 400 mL of Sucrose Solution. Dissect the tissue
and store at 4°C. 3. 2M TEAB stock: To make 500 ml, take 138.3 ml (101 g) TEA and fill to 400 ml with DDW. Use dry ice to bubble in CO2 until pH is below 8.0. Complete to 500 ml with DDW and store at 4°C. *TEA is extremely hazardous so take care
buffer (0.1 M Na2CO3 sodium carbonate, pH 9.0): To make 100 ml, weigh 1.06 g Na2CO3 and complete to 100 ml with DDW. Adjust pH to 9.0 by adding 10N NaOH and store at 4°C. 3. 2M TEAB stock: To make 500 ml, take 138.3 ml (101 g) TEA
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