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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°-20°C
- 保质期:
1-3年
- 库存:
999
- 供应商:
亦高生物
- 规格:
H4416-100ML

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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
EGTA stock, pH 8.0) in 100 ml PBS, pH 7.2-7.4 Heat about 80ml H2 O to 600 C and add paraformaldehyde; add 1-2 drops of 10M NaOH to get paraformaldehyde in solution. Cool to room temperature, add 10 ml 10X PBS, adjust pH with HCl, add MgCl
Cryopreservation of cell cultures
). 2. Remove the growth medium and wash twice in PBS (B0.1 ml cm2). 3. Add prewarmed (37 1C) trypsin solution (0.5 ml per 25 cm2) to cover the cell monolayer, recap the flask and incubate atB37 1C for 5 min. 4. Examine the monolayer and tap
%, 70%, 80%, 90%, 95%, and 100% alcohols for about two hours each. The blocks are then placed in a second 100% ethanol solution to ensure that all water is removed. Note that ethanol is hydroscopic and absorbs water vapor from the air. Absolute ethanol
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