Sartorius赛多利斯SDLV-0150-02E0-ES

v468 Chapter 2 Wnt活性蛋白分离及应用

(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl

Northern Blotting (Howell Lab)

℃, then chill on ice for 5 min. e) Add 2 µl loading buffer and 1 µl 0.4 mg/ml EtBr. For the ladder lanes, use 3 µg of 1 kb ladder and treat the same way as with the samples. Running the gel For the Hoefer, run

Northern Blotting (Breeden Lab)

), add same vol. loading buffer add 0.5 µl EtBr (0.5 µg/µl) heat for 5 min @ 90 °C , cool on ice III. Gel run run gel (8 x 10 cm) in fume hood with 70-100 V (-> 50-70 mA) run