Sartorius赛多利斯16508----------BP

In Situ HYBRIDIZATION TO TISSUE SECTIONS

ml glass tube -Spot 10ml on a GF/C filter disk -To the remaining 90ml, add 5ml cold 10% TCA; incubate 10' on ice -Filter onto GF/C, wash with 10% TCA, wash with 95% EtOH and dry -20-50% incorporation is good and corresponds to a S.A. ‰ 1-2 x 108

In Situ Hybridization Protocols

20mM Tris pH7.5 100mM NaCl PK Buffer: 50mM Tris-HCl pH 7.5 5mM EDTA 50mM Tris-HCl pH 7.5 5mM

Anderson Lab In Situ Hybridization Protocols

20mM EDTA 20mM Tris pH7.5 100mM NaCl PK Buffer: 50mM Tris-HCl pH 7.5 5mM EDTA 50mM