sartorius赛多利斯6981002不锈钢过滤漏斗Fun

Mag-Binds Plant DNA Kit Magnetic 96 Protocol

isolation protocol. Mechanical tissue disruption: Place sample into a stainless steel grinding jar with appropriate steel beads. Freeze samples in the stainless steel grinding jar using liquid nitrogen for 1 minute. Immediately attach

Isolation of bone marrow

syringe with ice-cold PBS. 3. Fit a blunted 21 gauge needle (nip the bevel off with pliers). 4. Squirt about 3ml of the ice-cold PBS down the shaft of each excised femur. 5. Catch the tissue fragments in a hemispherical 80-mesh stainless steel sieve

Isolation of bone marrow

stainless steel sieve perched on top of a plastic tube. 6. Rub the tissue fragments through the sieve using the bottom of another plastic tube, and rinse through with the remaining ice-cold PBS. 7. Centrifuge the sample. Isolation of bone marrow