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大量
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一年
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科邦兴业(北京)科技有限公司
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sartorius赛多利斯6981002不锈钢过滤漏斗Funnel 500 ml, stainless steel
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文献和实验Mag-Binds Plant DNA Kit Magnetic 96 Protocol
isolation protocol. Mechanical tissue disruption: Place sample into a stainless steel grinding jar with appropriate steel beads. Freeze samples in the stainless steel grinding jar using liquid nitrogen for 1 minute. Immediately attach
syringe with ice-cold PBS. 3. Fit a blunted 21 gauge needle (nip the bevel off with pliers). 4. Squirt about 3ml of the ice-cold PBS down the shaft of each excised femur. 5. Catch the tissue fragments in a hemispherical 80-mesh stainless steel sieve
stainless steel sieve perched on top of a plastic tube. 6. Rub the tissue fragments through the sieve using the bottom of another plastic tube, and rinse through with the remaining ice-cold PBS. 7. Centrifuge the sample. Isolation of bone marrow
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