赛默飞世尔Thermo Fisher PIPET SHORT

Procedures for In Vitro Production of Bovine Embryos

with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap

Northern Blotting方法(Howell Lab)

for at least 30 min before quantitation. Pipet up and down several times before taking 2 µl into 998 µl water. Read at 260 nm. RNA concentration (µg/µl) = OD x 500 x 0.04 µg/µl = OD x 20 Formaldehyde gel Boil 2 g agarose in 144 ml

In Situ HYBRIDIZATION TO TISSUE SECTIONS

2O. Pronase treat: -Remove the slides from the rack and place horizontally in a tray. -With a Pasteur pipet, carefully drip ‰0.25mg/ml pronase solution onto the sections and incubate 10' (thhe incubation time starts with treatment