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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
solution ( 36.5%; Sigma-Aldrich 33220) Glycine (2 M; Fisher BP381) Glycogen (Roche) H2 O, nuclease-free Immune complex wash buffers (high-salt and low-salt) Prepare the high-salt and low-salt versions of this buffer
Introduction to the EMSA (Gel Shift) Technique
and determining protein:DNA interactions is the electrophoretic mobility shift assay (EMSA). The EMSA technique is based on the observation that protein:DNA complexes migrate more slowly than free DNA molecules when subjected to non-denaturing polyacrylamide
2.Quantitate Protein Concentration Quantitate proteins in 15 ml aliquots of lysates (in duplicate)by using a Bradford assay (I have been using the Biorad kit),using BSA as standard.Don't forget to add 15 ml LB to each BSA sample and to the blank tube
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