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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70119-087980
中文名称:
英文名称:GLASS FILTER 2X4 1/2 SHADE 11
货期:热销品现货,其他请咨询




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文献和实验1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
, which will compress the gel, and retard the capillary transfer. Transfer overnight. 11. After transfer, separate the membrane from the gel and rinse the membrane briefly in 2X SSC buffer. Place the filter between two sheets of Whatman paper and bake at 80℃
Culturing Chick Embryonic Myoblasts/Myotubes and Fibroblasts
. 7. Ethanol (70% in a spray bottle and 95%). 8. Two sets of sterile glass petri dishes, sitting on ice in a plastic pan. 9. Two petri dishes with gelatin solution, prepared on day 1. 10. Nitex filter (20 um), preassembled by tying
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