赛默飞世尔Thermo Fisher COND STD, 7

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Stripping Western Blots

, harvest 24-48 hrs post transfection. LipofectAMINE Transfection of NIH3T3 Cells 1. Plate 3T3 cells at 2 x 105 per 35 mm dish & let grow overnight (cells should be 50-70% confluent for transfection). 2. For each transfection, dilute: A: 8 μl

重组DNA的分离、克隆与测序实验手册

[[gamma]]-[32-P]-rATP 10 uCiT4 polynucleotide kinase 1 μl (3U/ul)10 μl [[gamma]]-[32-P]-rATP (35020) ICN and T4 polynucleotide kinase (70031) from United States Biochemicals.2. Incubate at 37℃ for 30-60 minutes. 3. Heat the reaction at 65℃ for 10 minutes