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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
volume of average-sized sample. Add 0.5 vol. 8 M LiCl, mix and stand at 4°C overnight. 10. Spin at 12000g, 4°C, for 15 min in a fixed angle rotor. Thoroughly resuspend pellet in 4 ml sterile gd (glass distilled) H2 O with pasteur pipette. 11. Extract twice
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Dissecting microscope; Acid Tyrode's (e.g. Sigma: T1788); Sterile plastic Petri dishes (100 x 15 mm); M2 and KSOM medium in 1 ml syringes; 9'' Pasteur pipettes; Mouth or Finger controlled pipette. Method: Place
Clone Genes From a Phage Library
, you need to use a seal-a-meal bag, with about 1.5 to 2 ml hybe solution per small plate-sized filter. (I use 20 ml for 12 filters) • Put to 65 degrees for 3 hours to overnight or longer, with sufficient agitation to expose all the filters
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