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货号:T_70114-828-90
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英文名称:BG BIO RED 1.75ML 38X45 125/CS
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文献和实验Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
Klein, T. M. (1990) Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio-Technology8, 833-839. 8. Somers, D. A., Rines, H. W ., Gu, W ., Kaeppler, H. F. and Bushnell, W. R. (1992)
In Situ HYBRIDIZATION TO TISSUE SECTIONS
1.0ml 10mM rGTP 1.0ml 0.5mM rUTP 1.0ml 0.75M DTT 1.0ml RNase-Block (25U/ml) 2.5ml 35S-rUTP (100mCi) 1.0ml T3 or T7 polymerase (10U) Vortex, spin down and incubate 1-2h @ 30 degrees C. Incorporation should be 70-90%. Determine
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