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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310729311
中文名称:
英文名称:701 N 10µL Grob(.23/75/45°) length, 10µL
货期:热销品现货,其他请咨询




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文献和实验-stop PCR only; see Steps 1-22)SSCP loading dye (for SSCP only; see Steps 23-37)95% (v/v) formamide10 mM NaOH0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanolTaq DNA polymerase (5 U/µl)TBE Buffer (1X and 5X)Prepare a 5x stock solution in 1 liter
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
high background, but does not make the length of chromatin DNA short enough for ChIP. (10) Centrifuge for 20 min at 14000 rpm, 4°C. Recover supernatant. Do not take yolk proteins precipitated in the bottom of the tube. (11) Add 55.6 µl of 10
DpnI mediated site-directed Mutagenesis
, 68°C 2 minutes/kb of plasmid length 3. Degradation of methylated (parental) DNA with DpnI Cool down PCR reaction. Add 1µl Dpn I (10 unit) to PCR reaction 37°C and incubate for 1 hr. 4. Transformation into E. coli
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