赛默飞世尔Thermo Fisher ACETIC ACID

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

) Chloroform (CHCl 3 , Fisher) Acetic acid (AcOH, Acros) Silica gel (60 Å, 230 to 400 mesh, Merck

单组分溶液配制

. Name: Formula: Mw % (w/w) [M] g in 1L of subst. p [g/ml] ml/L for 1M sol. Sodium hydroxide NaOH 40 50% 19.1 763 1.53 52.4 30.1% 10.0 400 1.329 100 10% 2.75 111 1.11 363.6 Potassium hydroxide KOH 56.1 50% 13.5 757 1.52

SDS-PAGE

) protein, load only 0.0125 OD ml. For Drosophila tissue culture cells, suspend 3X106 cells per 90 µl cracking buffer, and load about 20 µl per lane (0.8 mm thick gel with the BRL gel box and 20 well comb). 10. Running. Use a constant Amps power supply