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货号：T_70110-437-30B
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
is thus desirable. Such a system has been developed for isola-ting plant RNA (Nagy et al. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora
【转贴】immunofluorescence staining
Ca++ and Mg++). For "Whole fixation" fix the cells with 4% Formaldehyde (EM grade only) in PEM buffer for 30 min. on ice (1:4 dilution of 16% stock).The fixation buffer should well cover the surface of the cells so that they do not dry out (about 250
(Fisher) Heating mantles (VWR) 100‐, 250‐, and 2000‐mL Erlenmeyer flasks (Kimax
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