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999
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北京泽平
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货号：T_701R72742004A
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英文名称：SODIUM HYDROXIDE, 32% W/V, 4 L
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
lysis under a microscope.5. Pellet the nuclei at 2,000 X g for 10 min at 4℃B. Run-On Transcription Reaction1. Resuspend the nuclei in 150 2X Transcription Mix and 30 μl 10X Nucleotide Mix, 500 μCi [32P ]-rUTP, and bring the final reaction volume to 300 μ
on ice for 5 min and check for cell lysis under a microscope. 5. Pellet the nuclei at 2,000 X g for 10 min at 4℃ B. Run-On Transcription Reaction 1. Resuspend the nuclei in 150 2X Transcription Mix and 30 μl 10X Nucleotide Mix, 500 μCi [32
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