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货号：T_70311729148
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文献和实验Co-Expression of human DNA primase p49-p58 subunits
if you can do all of the spin downs in refridgerated centrifuges.If you can't do this,then work quickly and keep all buffers ice cold. Lysis buffer: •1% Triton X-100 •50mM Tris pH 8.0 •150mM NaCl •20mM imidazole (to decrease non-specific binding of proteins to Ni beads
Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
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