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文献和实验of an ocular grid inserted into an inverted phase contrast microscope. Cell density (cells/cm2) is then plotted on a log scale against time in culture.Exercise 12.1 - Aseptic Cell Transfers Level I Figure 12.3 Flaming a wire loop and removing cap
Establishment of a Primary Culture
in MEM at 1000 rpm for 10 minutes in a standard clinical centrifuge. Remove the supernatant and resuspend the pellet in 25 ml of fresh MEM + 10% FCS. Remove 0.1 ml of the culture and determine cell concentration and viability
. No. D8537) using a volume equivalent to half the volume of culture medium. Repeat this wash step if the cells are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area
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