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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
RNase Protection Assay(RPA)操作步骤
相关专题 核糖核酸酶保护实验 The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA
A Homogeneous Assay to Assess GABA Transporter Activity
a convenient functional uptake assay for GABA transport into cell lines transiently transfected with GABA transporter?1 (GAT?1) and other GAT isoforms. This facile, homogeneous assay allows for the determination of K m , V max , and K i values. The assay
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