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英文名称:X50 BOTTLE RD NM HDPE IP2 1000ML PP-Verschluss 38-430, VE=50 Stück
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文献和实验A safe method of extracting DNA from Coccidioides immitis
in the saprophytic phase at 37 C in 100 ml of 2X GYE medium (20 g glucose and 10 g yeast extract in 1 liter medium, autoclaved for 15 min.) in 500 ml plastic Nalgene flasks with plastic foam stoppers (Identi-plug, Fisher). When there has been sufficient growth
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
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