赛默飞世尔Thermo Fisher Evaporating

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

of CTO stock solution into 18 ml of serum-free MCDB-131 medium.     4)      Rinse EPCs in 3 near confluent T-75 cell culture flasks with 10 ml DPBS (without Ca or Mg).     5)      Add 6 ml of the 2 μM CTO working solution to each flask

Bel-7402 人肝癌细胞系

at 1000rpm for 10min, than washed two times with cold PBS by centrifug ation (1000rpm, 5min), resuspended in 0.5mL PBS, adjusted to a cell concentration of 1×106/L, and fixed with ice-cold 70% alcohol at 4℃ (could the preserved for less than 2 weeks

Supported Membrane Formation, Characterization, Functionalization, and Patterning for Application in Biological Science and Technology

; see for selection] Deionized water (resistivity ≥18 MΩ and apparent pH 5.5) Argon 25‐ to 50‐ml glass round‐bottomed flask (cleaned by piranha or base etching; see protocol