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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70315135624
中文名称：
英文名称：125ML CX-1 pH Gradient Buffer B (pH 10.2)
货期：热销品现货，其他请咨询
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文献和实验Immobilized pH gradient (IPG) as first dimension
chamber using 3 ml per groove of buffer 1 (50 mM Tris-HCl pH 6.8, 6 M urea, 30% v/v glycerol, 2% w/v SDS and 2% w/v DTE), and then -SH groups were subsequently blocked for 5 min using buffer 2 (50 mM Tris-HCl pH 6.8, 6 M urea, 30% v/v glycerol, 2% w/v SDS
Sucrose Density Gradient Fractionation
about 1Add 1 ml of COLD 1M NaN3 to 100 ml culture (we want 10 mM total conc.)Spin down 3 x109 cells (about 100 O.D.equivalents (100 ml of OD = 1): 2750 X g.,10 min.,30℃ in lab clinical centrifugeWash 1x with 10 ml SK buffer in 15 ml falcon tubes (see recipe
after use), combine to make 5 ml of 10X reaction buffers, put 1 ml in to each of 5 microfuge tubes (MFTs) and store the current one at -20℃ and the rest at -70℃.A: 1 M Tris-HCl 50 ml: 4.88 g Tris-HCl + 2.3 g Tris-Base (pH 7.9 at 25℃)B: 1 M Tris-HCl 50 ml
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