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货号:T_70310381332
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英文名称:X50 BOROSILICATE TUBE 25 ML CYL Borosilikatglas, VE=50 Stück
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
5M stock 1.5 ml Fill up to a total volume of 50 ml with H2 O (8) Homogenize tissues on ice (10-12 strokes). Transfer the homogenate into a microfuge tube on ice.
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
A quick RNA mini-prep for Neurospora mycelial cultures
and, potentially, other filamentous fungi. Below, we describe the use of this procedure with 50 ml mycelial cultures, although we have used in with equal success with 5 ml cultures without scaling down the amounts of any reagents. The method involves the use
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