赛默飞世尔Thermo Fisher 透明玻璃培养管，12

Basic procedures for bacteria culture

LB 2. Pick several colonies into a 12 X 75 mm Falcon tube containing a 2 ml aliquot of the respective liquid media, and incubate for 8-10 hours at 37degC with shaking at 250 rpm. 3. Transfer the 2 ml culture into an Ehrlenmeyer flask

Differentiating Neural Stem Cells into Neurons and Glial Cells

of the culture vessel with the working solution of CELLStart™ CTS® (14 mL for T-75, 7 mL for T-25, 3.5 mL for 60-mm dish, 2 mL for 35-mm dish).        c. Incubate the culture vessel at 37°C in a humidified atmosphere of 5% CO2 in air for 1 hour.      

RNA purification using TRizol--TRIZOL法提取RNA

of chloroform to each tube.b.Cap each tube. Shake samples vigorously by hand for 15 seconds.c.Incubate samples for 5 minutes at room temperature.d.Centrifuge samples for 15 minutes at 12,000 x g at 4oC.3.  RNA Precipitation a.Transfer the upper aqueous phase