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- T_70122-022936
- 2025年07月08日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70122-022936
中文名称：
英文名称：CELL SAMPLE F/ PHILLIPS 100/PK
货期：热销品现货，其他请咨询
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Dissecting microscope; Acid Tyrode's (e.g. Sigma: T1788); Sterile plastic Petri dishes (100 x 15 mm); M2 and KSOM medium in 1 ml syringes; 9'' Pasteur pipettes; Mouth or Finger controlled pipette. Method: Place
Cell counting with an hemacytometer.
Inc. Garden grove CA 92841 Hemacytometer Fisher scientific No 02671 5 Dilute your cell sample in Trypan Blue dye exclusion medium. Dead cells will be blue, live cells will be birefringent. Suggested dilution 1:20 (5µl cell suspension in 95 µl Trypan Blue
sample to equilibrate and analyze for fluorescence immediately. top of page REAGENTS:Solution A:0.1 % Triton X-100, 0.08N HCl, 0.15M NaClSolution B:6 ug/ml Acridine Orange [Sigma, A 6014] , 1 mM EDTA-Na, 0.15M NaCl, 0.2M NaPO
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