12 x 75 mm Polypropylene Cultu

PC12 Cell Culture and Fusion

ECL matrix. 5. Coverslip dishes. Add 12 ml of cold (4oC) F12K medium to a tube of ECL aliquot, vortex. Pipet 2-3 ml on each coverslip dish and incubate for at least 1 hr in the incubator. Replace the ECL solution with culture medium before use

ES CELL DNA EXTRACTION: TUBE ME

in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this way! Lysis buffer: ( 100 ml Recipe ) 10 mM Tris-HCl pH7.5 - 0.5 ml of 2M 10 mM EDTA - 2 ml of 0.5M 10 mM NaCl - 0.2 ml

E. Z. N.A.TM X-press Plasmid Protocol

) into the Xpress spin column. 11. Centrifuge at maximum speed (13,000 x g) for 1 minute. 12. Discard the flow-through liquid and re-use the collection tube. 13. Place the empty X-press spin column back into the 2 ml collection tube. Centrifuge