- ￥4500
- Sino Biological
- 北京
- 40591-V02H3
- 2025年08月12日
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- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant SARS-CoV-2(2019-nCoV)Spike S1(D614G),ECD-Fc Recombinant Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
纯度> 90 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag)(10108-H08H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1(D614G), ECD-Fc(40591-V02H3), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1(D614G),ECD-Fc(40591-V02H3) is 40-230 ng/mL.
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-Fc Recombinant Protein (YP_009724390.1) (Val16-Arg685(D614G)) was expressed with the Fc region of human IgG1 at the C-terminus.
NP No.YP_009724390.1
表达宿主HEK293 Cells
种属2019-nCoV
预测 N 端Val 16
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-Fc Recombinant Protein consists of 908 amino acids and predicts a molecular mass of 101.7 kDa.
缓冲液Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验抗体等。Mabselect、Protein G和Protein A对IgG的Fc区有专一性亲和作用，能一步纯化各种不同源的IgG。血清互补剂如小牛血清可先用蛋白G预处理，在培养前除去IgG。重组蛋白A介质Mabselect和rProtein A Sepharose FF对IgG有更高的载量和专一性，基团脱落更少。脱落的rProtein A用离子交换Q Sepharose HP或凝胶过滤Superdex 200，很容易去除。 *疏水层析Phenyl Sepharose HP亦很适合纯化IgG
生物分子下游纯化的对象一般包括蛋白、酶、重组蛋白、单抗、抗体及抗原、肽类、病毒、核酸等。纯化前首先需要测定生物分子的各物理和化学特性，然后通过实验选择出最有效的纯化流程。1.测定------分子量、PI当目标蛋白的物理特性如分子量、PI等都不清楚时，可用PAGE电泳方法或层析方法加以测定。分离范围广阔的Superose HR预装柱很适合测定未知蛋白的分子量。用少量离子交换介质在多个含不同PH缓冲液的试管中，可简易地测出PI，并选择纯化用缓冲液的最佳PH。2.选择------层析方法若对目标蛋白
生物分子下游纯化的对象一般包括蛋白、酶、重组蛋白、单抗、抗体及抗原、肽类、病毒、核酸等。纯化前首先需要测定生物分子的各物理和化学特性，然后通过实验选择出最有效的纯化流程。1、测定——分子量、PI当目标蛋白的物理特性如分子量、PI等都不清楚时，可用PAGE电泳方法或层析方法加以测定。分离范围广阔的Superose HR预装柱很适合测定未知蛋白的分子量。用少量离子交换介质在多个含不同PH缓冲液的试管中，可简易地测出PI，并选择纯化用缓冲液的最佳PH。2、选择——层析方法若对目标蛋白的特性或样品成分
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