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货号:T_70310607331
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英文名称:Jar with snap-on lid PE-LD 18 ml CAPS 18ML (PACK OF 12)
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
When you are ready to cast the fibrin gel construct, prep a 1 mL syringe with a 18G 1 ½ inch long needle. Have a 21G 1 inch needle ready as well. 4) The fibrin solution is created at a 4:1:1 ratio of F solution: T solution: cell solution. To make one mL
ng/μl for pUC templates) into the bottom of the 0.2 ml thin-walled reaction tubes (Robbins Scientific). Use the 1 μl sample for A and C reactions, and the 2 μl sample for G and T reactions. Meanwhile, preheat the PE Cetus Thermocycler 9600 to 95℃
-HCl pH 8.5 (dilute from 1 M stock), 1.5 mM MgCl2 (dilute from 1 M stock), 0.2 mM of each dNTP (dilute from 100 mM stock), and 5 units of PE-Cetus Amplitaq in 0.5 ml snap cap tubes for 25 cycles of 95oC for 1 min., 55oC for 1 min. and 72oC for 2 min
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