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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
1年
- 规格:
EA
货号:T_70310480421
中文名称:
英文名称:Jar with snap-on lid PE-LD 5 ml CAPS 5ML (PACK OF 12)
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
in 20 mL of DMEM to be counted using a hemocytometer, and place the cells on ice. 12) Place 50 μl of the cells into a Trypan Blue solution (75 μl Trypan Blue, 125 μl PBS), mix well before placing 10 μl in the hemocytometer
ng/μl for pUC templates) into the bottom of the 0.2 ml thin-walled reaction tubes (Robbins Scientific). Use the 1 μl sample for A and C reactions, and the 2 μl sample for G and T reactions. Meanwhile, preheat the PE Cetus Thermocycler 9600 to 95℃
-HCl pH 8.5 (dilute from 1 M stock), 1.5 mM MgCl2 (dilute from 1 M stock), 0.2 mM of each dNTP (dilute from 100 mM stock), and 5 units of PE-Cetus Amplitaq in 0.5 ml snap cap tubes for 25 cycles of 95oC for 1 min., 55oC for 1 min. and 72oC for 2 min
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