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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(E484K)-His Recombinant Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100.00 µg
纯度> 90 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(E484K)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(E484K)) was expressed with a polyhistidine tag at the C-terminus.
表达宿主HEK293 Cells
种属SARS-CoV-2
预测 N 端Arg 319
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike S1(Arg319-Phe541(E484K))-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.5 kDa. As a result of glycosylation, it migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验2, Lee, Y; et al. SARS-CoV-2 mRNA vaccine induced higher antibody affinity and IgG titers against variants of concern in post-partum vs non-post-partum women. EBioMedicine, PMID: 35301181
3, Shim, JE; et al. Single-Nanoparticle-Based Digital SERS Sensing Platform for the Accurate Quantitative Detection of SARS-CoV-2. ACS applied materials & interfaces, PMID: 35951983
4, Tai, W; et al. An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variants. Nature communications, PMID: 37221158
日前,北京大学生命科学学院魏文胜课题组在 Cell 杂志上在线发表题为“Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants”的研究论文。 魏文胜团队首先建立了体外高效制备高纯度环状 RNA 的技术平台,针对新型冠状病毒及其变异株,设计了编码新冠病毒刺突蛋白(Spike)受体结构域(RBD)的环状 RNA 疫苗。 该项研究中制备的针对新冠病毒德尔塔变异株的环状 RNA 疫苗(circRNARBD
三句话读懂一篇 CNS:新冠病毒或导致男性过度免疫反应;间歇性禁食能够延寿并降低多种疾病风险
to time-restricted feeding in mammals。 该研究在不同的时间从实验组和对照组小鼠的 22 个器官中收集组织样本,发现间歇性禁食影响着小鼠近 80% 的组织部位的基因表达,可促进寿命延长,并降低糖尿病、心脏病、高血压和癌症等风险。 图 1:来源 Cell Metabolism 2. Nature:新冠病毒可能导致男性过度免疫反应 自 2019 年新冠病毒的爆发和肆虐以来,给人们的正常生活与身心健康带来了巨大的负面影响。 2023 年 1 月 4 日,美国国家过敏和传染
冠状病毒,即使穿山甲最后被证明是中间宿主,也不能说上述研究中用到的马来亚穿山甲是新型冠状病毒的中间宿主。新冠病毒溯源是否还有另外一种可能?在另外一篇新型冠状病毒溯源的研究中,我们发现了新型冠状病毒起源与进化的部分线索。新型冠状病毒与 MERS 冠状病毒、蝙蝠 SARS 冠状病毒和 SARS 冠状病毒处于相同的乙型冠状病毒进化枝中。与蝙蝠 SARS 冠状病毒平行进化,相比于 SARS 冠状病毒,新型冠状病毒与蝙蝠冠状病毒存在更密切的亲缘关系。图源:Cell Host & Microbe 官网2 月 22 日,南方
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