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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
Baerga‐Ortiz, A., Hughes, C.A., Mandell, J.G., and Komives, E.A. 2002. Epitope mapping of a monoclonal antibody against human thrombin by H/D‐exchange mass spectrometry reveals selection of a diverse sequence in a highly conserved protein
Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
Figure 13.11.4 Multiplexed flow cytometry analysis during monoclonal antibody development. (A ) Multiplexed titer analysis of sera from a representative mouse challenged with RGS19. (B ) Representative single‐point multiplexed hybridoma
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