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- 2025年07月08日
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100 mg
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
fresh 10 mg/ml stock in DMSO.Need 2.5 mg per 50 mg of tubulin. 8.Potassium glutamate,1 M.Prepare 1 M glutamic acid and titrate with KOH to pH 6.9. 9.Injection Buffer: 50 mM potassium glutamate,0.5 mM MgCl2,pH 6.5-6.7.Prepare 10 ml.Keep ~5 ml at 37℃
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,1 mM PIPES,pH 6.8 at 4℃,250 ml for day 0. 2.0.5 mM ATP,0.2 mM CaCl2,70 mM KCl,150 mM PIPES,pH 7.0,1 ml. 3.16 mM ATP,0.2 mM CaCl2,5 mM DTT,5 mM lysine,10 mM glutamic acid,20 mM Tris-HCl,pH 8.0,10 ml
Preparation Of Peptide-KLH Conjugates For Immunization
.(6) For the -SH coupling, warm the other aliquot of KLH to room temp. Add 1/9 th volume of Iodoacetic acid N-hydroxysuccinimide ester at 100mg/ml in DMSO. Make the DMSO stock fresh, and protect the iodoacetamide reagent from light. We make our own IAA-NHS ester
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