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文献和实验Synthesis and Application of Highly Reactive Amino Linkers for Functional Oligonucleotides
are functionalized by conjugation with a variety of molecules, and aliphatic amino linkers have been frequently used as a tether for their modifications. This unit describes the syntheses and applications of novel amino linkers having a carbamate structure
Coupling Peptides To Resin For Affinity Purification
volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add
Immunofluorescence on ultrathin resin sections
. Residual water is drained with filter paper along the outside of the loop. Note: air-dried resin sections can be stored for months prior to labelling. Procedure: Encircle sections
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