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- ProteoChem
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- 2025年07月10日
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文献和实验Exonuclease III Footprinting on Immobilized DNA Templates
is a suitable probe to analyze the boundaries of a protein when it is necessary to eliminate any excess unbound DNA from the reaction to avoid background problems. In combination with biotin-labeled DNA that is bound to streptavidin-coated magnetic particles
Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads
during the amplification reaction. The DNA can then be immobilized onto streptavidin-coated paramagnetic beads, simultaneously removing buffers, dNTPs, and unincorporated PCR primers. By alkali denaturation, the immobilized double-stranded DNA is converted into a single
Immobilized pH gradient (IPG) as first dimension
Racket-shaped IPG A new sigmoidal immobilized pH gradient (IPG) was used as the first dimension. It offered high resolution and great reproducibility and allowed high protein loads. Based on our specifications, the non-linear pH gradient
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