- 询价
- ProteoChem
- LC6312-1L
- 2025年07月15日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 规格：
1 L
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验In-gel Tryptic Digest for Protein ID by Mass Spectrometry
of 12.5 ng/μl trypsin (Promega sequence-grade modified porcine trypsin,Cat.#V511A)in 50 mM NH4HCO3.To make the digestion buffer,dissolve 20 µg Promega trypsin in 80 μl Promega trypsin buffer solution (50 mM acetic acid),and dilute with 50 mM NH4HCO3
In-gel digestion of proteins for peptide fingerprint mapping
the final volume of the extracts to ~10 m l in a vacuum centrifuge and add 5 m l 5% Formic acid/ 50% acetonitrile. 10. Store the recovered peptides at -20 °C in slick tubes. References Fenselau, C. 1997. MALDI-MS and strategies
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
L ) Spin down liquid and transfer the 'basic extracted' peptide containing solution to the same tube as the 'digest solution' Add 40-50 µl of 5% formic acid (to adjust the pH) Incubate for 15 min at 37°C while shaking Spin
技术资料暂无技术资料 索取技术资料
