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文献和实验both at transcriptional or posttranscriptional levels (8 -10 ). Recombinant fusion proteins consisting of various bacterial toxins selectively targeted to the tumor by virtue of single-chain anti-erbB- 2 antibody (sFv) moieties has also been utilized in this context (7 ).
c-erbB-2 Immunohistochemistry in Paraffin Tumors
Immunohistochemistry is the study of the intracellular distribution of antigens based on the formation of an immune complex. The concept is based on the application of a specific antibody to the antigen to be detected and visualization
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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