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文献和实验Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
. Kritzer, J.A., Luedtke, N.W., Harker, E.A., and Schepartz, A. 2005. A rapid library screen for tailoring beta‐peptide structure and function. J. Am. Chem. Soc. 127:14584‐14585
Antigen Design Sera Purification Tech Sheet
. This can be determined by performing a competitive peptide blocking study. Peptide blocking studies check that the response against the target protein is not a background artifact. Ammonium Sulfate Precipitation Ammonium sulfate precipitation is a commonly used
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