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文献和实验Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
INTRODUCTION Flow cytometry is frequently used to assess nucleic acid content in individual cells. Based on DNA content alone, however, cells in the quiescent G0 phase cannot be discriminated from cells in the proliferative G1 phase
rates of 1, 2μmol/L arsenic trioxide were 19.10% and 21.87% respectively, which were much higher (both P＜0.05). Decrease of G0/G1 phase cells and increase of S phase cells were observed by flow cytometry, suggesting the inhibition effect of 0.5, 1, 2μmol
A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
MATERIALS : 1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide) 2. 7-Amino-actinomycin D (7-AAD, e.g., Calbiochem, CA) 3. Human AB serum, heat-inactivated
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