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- 文献和实验
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- 库存：
999
- 规格：
100 ug
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文献和实验8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
Flow Cytometry Assay to Measure Internalization of GPCR
chelating agents. 8) Label receptors with fluorescent antibody -resuspend pellet in 500 µL of media containing 10 mg/mL FITC-M1 (M1 which has been covalently bound to the FITC fluorophore ( Molecular Probes ) using a standard procedure) - incubate 45
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