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文献和实验Assays of Glucose Entry, Glucose Transporter Amount, and Translocation
and other hormones (1 ,2 ). Indeed, in those tissues, glucose transporter 4 (GLUT4) is the chief isoform which is, in basal conditions, retained in a specific intracellular storage compartment (3 ). The GLUT4-containing vesicles are translocated to the plasma
Study of Glucose Uptake in Adipose Cells
. One notable exception is the glucose transporter isoform 4 (Glut4), which is specifically expressed in insulin sensitive tissues, i.e., skeletal muscle, heart muscle, and fat, and is responsible for the insulin effect on blood glucose clearance
® XL PCR Cloning Kit •High-efficiency cloning of long (3-10kb) PCR products K4700-10 •Positive selection resulting in low background TOPO PCR表达克隆法 传统限制酶切法必须把目标片段切下来再连接至表达
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