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文献和实验Transporter Current Measurements
Na+ /K+ pump, Na+ /Ca2+ exchanger, glutamate transporter and other transporters for dopamine, norepinephrine, GABA, and others, are all electrogenic. These transporters or exchanger transport Na+ ions, with or without transmitters
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
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