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文献和实验Measurement of Phosphoinositide 3-Kinase Activity
only PtdIns as a substrate (e.g., mammalian PtdIns 3-kinase and yeast Vps34p) ( 1 ). Generally, PI 3 kinases are now regarded as an important intracellular signal upstream of a variety of biochemical (e.g., activation of Akt/protein kinase B and/or p70
DNA-Dependent Protein Kinase in Apoptosis
target proteins, one of which is the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs). The antiapoptotic factor Bcl-xL can sequester cytochrome C and inhibit the formation of the caspase-9-Apaf-1 complex effectively blocking apoptosis
Spatiotemporal Dynamics of Kinase Signaling Visualized by Targeted Reporters
Figure 1. Schematic diagram showing the modular structure of kinase activity reporters. Kinase activity reporters consist of a FRET donor (e.g., CFP), a phosphoamino acid binding domain (PBD), a consensus phosphorylation sequence (substrate peptide
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