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MCE 细胞线粒体分离试剂盒可快速分离所培养细胞的线粒体,且线粒体纯度较高,绝大多数具有完整的内外膜及生理功能。
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文献和实验Isolation of Centrosomes from Cultured Cells
from adherent cultured cells by density-gradient centrifugation. It further describes how to evaluate the activity and function of centrosomes by microtubule nucleation and immunofluorescence assays and how to measure microtubule dynamics nucleated from isolated
Isolation of Yeast Mitochondria
) from a desired organelle, these unwanted components often confuse the resulting data. For these types of studies, it is crucial to have highly pure fractions of the desired organelle. Here we describe a protocol for purification of mitochondria from Saccharomyces
Isolation and Purification of Functionally Intact Mitochondria from Plant Cells
The isolation of mitochondria from plant cells that display similar biochemical and morphological characteristics to those observed in vivo requires considerable expertise. Although it is relatively easy to prepare a crude mitochondrial
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