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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
of methods used to uncover insertion sites have previouslybeen described, including genomic DNA library screening(15), ligation-mediated PCR (6), inverse PCR (16), VISAtechnique (17), T-linker PCR (18), and single nucleotidepolymorphism-based mapping (19
【精华】vol 687 Chapter 5 PCR引物与PCR引物设计
., Wallace, I.M., Wilm, A., Lopez,R., Thompson, J.D., Gibson, T.J., and Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947–8. 19. iCODEHOP: Interactive Program for Creating Consensus-Degenerate Hybrid
Construction and Characterization of Adenovirus Vectors
. For large-scale preparation of Ad vectors using suspension-adapted 293N3S cells: ix. Distribute a 3-L culture of 293N3S cells into eight 500-mL centrifuge bottles. x. Centrifuge at 650g for 20
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