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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
. 10. Discard supernatant and wash with 70% ethanol. 11. Centrifuge at 2,500 × g for 10 min at 4°C to pellet genomicDNA again. 12. Discard supernatant and add 100–300 mL of TE buffer. 13. Incubate at 56°C for 30 min to let genomic DNA
【精华】vol 687 Chapter 5 PCR引物与PCR引物设计
of the targeted sequence. We have had the best success byusing the following steps in sequence: 1. Prepare eight PCR reaction mixtures on ice, each containing: 2.5 ul – 10× Gibco PCR buffer 1.0 ul – MgCl2 (50 mM) – 2 mM final concentration2.0 ml –
to an Ehrlenmeyer flask containing 50 ml of similar media, and incubate further for 8-10 hours. Transfer 12.5 ml of the culture to each of 4 liters of similar media, and incubate for an additional 8-10 hours. 2. Harvest the cells by centrifugation at 7000 rpm
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