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微型反式印迹模块是微型反式印迹细胞(目录号1703930)的变体。该模块配有两个凝胶盒、泡沫垫、电极组件和冷却装置,但没有缓冲罐或盖子。Mini Trans-Blot模块与Mini-PROTEAN Tetra(目录号1658000至1658007)完全兼容,可与Mini-PROTEAN系统的缓冲罐和盖子配对用于印迹转移。
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays
A variety of techniques can be used to find protein partners, including immunoprecipitation, affinity chromatography, blot overlays, and yeast twohybrid screening. Of these, the blot overlay protocol of sodium dodecyl sulfate (SDS)-acrylamide
Fast and reliable mini-prep RNA extraction from Neurospora crassa
from contaminating DNA, while clear nondegraded ribosomal bands were seen in all 200 extractions we made (see Figure 1). Northern blot analysis by hybridization with several 32P-labelled probes (Figure 2) and in vitro translation experiments (Figure 3) have shown
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