赛默飞世尔Thermo Fisher X960 0,5-10

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

. Shake rack thoroughly during the washing procedure. iv. Rinse slides in PBS. Add 100 µL of a solution containing 0.5 mg/mL DAB and 0.01% H2 O2 . v. To prevent overstaining, follow the appearance

High Throughput Isolation Of PCR Products Using ChargeSwitch® PCR Clean-Up

) 300 ml ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5) 48 ml 实验设备   96 x 200 µl U-bottomed microtiter plate Any liquid